Charles Knapp

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Contact Details

Dr Charles Knapp

senior lecturer

James Weir Building

charles.knapp@strath.ac.uk

Tel : +44 (0)141 548 3351

Profile

Research Interests

Microorganisms are omnipresent and capable of impacting the entire biosphere. They, especially the bacteria, are highly diverse in terms of structure and function, and they can play a major role in cycling of nutrients, remediation of contamination, and public health. Unfortunately, many people often overlook the ecological interactions within the microbial communities that support the process. Understanding these interactions require knowledge of the distribution and abundance of organisms and their interactions in an environmental setting. Further, it requires analytical tools to examine microbial organisms in an effective and timely fashion. Fortunately, high throughput culture-independent molecular methods are allowing researchers to quantitatively monitor these interactions. My research interest involves the integration of state-of-the-art microbiological measurement technologies and ecological principles into the realm of environmental protection and sustainability.

Microbial ecology and environmental microbiology research interests include:

  • Water quality 
  • Biological indicators of environmental pollution
  • Eco-toxicology
  • Bioremdiation
  • Outdoor, microcosm/mesocosm experimental systems
  • Microbiological community stability in engineered and natural systems
  • PPCP (pharmaceutical & personal care products) in the environment
  • Antibiotic resistance in the environment
  • Antimicrobial susceptibility assays
  • Algal/phytoplankton ecology / biotechnology

Academic / Professional qualifications

Work Experience

Education

  • Ph.D. Environmental Science, The University of Kansas, USA (2002)
  • MSc. Environmental Science (1999)
  • BSc.  Businesss Administration (1994)

Dr Charles Knapp

Senior Lecturer

charles.knapp@strath.ac.uk

Tel : +44 (0)141 548 3351 (Ext. 3351)

Microorganisms are omnipresent and capable of impacting the entire biosphere. They, especially the bacteria, are highly diverse in terms of structure and function, and they can play a major role in cycling of nutrients, remediation of contamination, and public health. Unfortunately, many people often overlook the ecological interactions within the microbial communities that support the process. Understanding these interactions require knowledge of the distribution and abundance of organisms and their interactions in an environmental setting. Further, it requires analytical tools to examine microbial organisms in an effective and timely fashion. Fortunately, high throughput culture-independent molecular methods are allowing researchers to quantitatively monitor these interactions. My research interest involves the integration of state-of-the-art microbiological measurement technologies and ecological principles into the realm of environmental protection and sustainability.

 

 

Qualifications

Work Experience

Education

  • Ph.D. Environmental Science, The University of Kansas, USA (2002)
  • MSc. Environmental Science (1999)
  • BSc.  Businesss Administration (1994)
  • Pg.Dip. Advanced Academic Studies (2013)
Research interests

Microbial ecology and environmental microbiology

  • Water quality determinations
  • Biological indicators of environmental pollution
  • Eco-toxicology, bioremediation
  • Outdoor, microcosm/mesocosm experimental systems
  • Microbiological community stability in engineered and natural systems
  • PPCP (pharmaceutical & personal care products) in the environment
  • Antibiotic/antimicrobial resistance in the environment
  • Antimicrobial susceptibility assays
  • Algal/phytoplankton ecology / biotechnology

Routine research services

  • DNA/RNA analyses
  • PCR (polymerase chain reaction)
  • Genetic probe design (primer design)
  • Community analysis (sequencing, DGGE)
  • Biological assessments of soil, sediment and water
  • Molecular and microbiological techniques
  • Phytoplankton, algae
  • Bacteria
  • Micro-invertebrates
  • Water quality monitoring
  • Soil quality determinations
  • Wastewater analysis
Esteem factors

Invited presentations/lectures (examples)

  • USA: IWA - MEWE conference (2013)
  • GermanyGerman Federal Environment Agency (UBA)
  • USA: Kansas State University (2013)
  • USA: U. Wisconsin-Milwaukee (2013)
  • UK: U. Stirling (2012)
  • UK: NHS-Scotland (2012)
  • UK: Robert Gordon U., Aberdeen (2012)
  • Canada: U. Manitoba (Faculty Honour Lecture) (2011)
  • Canada: U. Winnipeg, Dept. Chemistry  (2011)
  • UK: U. Newcastle, Civil Engineering & Geosciences (2009)
  • UK: U. Glasgow, Civil Engineering (2009)
  • UK: U. Edinburgh, Civil Engineering (2009)
  • USA: U. South Carolina, School for the Environment (2008)
  • USA: Troy U., School of Biological Sciences (2008)
  • Austria: Universitat Wien, Dept. fur Mikrobielle Okologie (2007)

Associate Editor

Editorial Board memberships

Peer Review College memberships

Expertise/panel memberships 

  • PREPARE policy (U. Oxford; 2009*) 
  • United Nations GESAMP (Group of Experts on the Scientific Aspects of Marine Environmental Protection) (2010-current)

Routine Peer Reviewer (other than as editor)

Media coverage 

Recent awards & recognition

 

Recent funding and projects

Project groups 

  • Franken-soils (2010 - current) - land remediation/restoration
  • League of Villains (2009 - current) - antimicrobial resistance/pathogens
  • The ARGs...  (2009 - current) - antimicrobial resistance (on-going series with U. Manitoba/Winnipeg)
  • Orpheus (2011-2012) - ultrasound technology
  • Bug-free (2009-2011) - skin/prosthetic microbial communities

Research

  • 2013 - Carnegie Summer Vacation Bursary: "Microbial community MIC determinations"
  • 2012 - Bridging the Gap, with collaboration with Prof. M. Federev & N. Hunt (Physics), Prof. R. Ulijn (Chemistry), Dr. A. Mackenzie (AMRL): "Reconfigurable nanomaterials: a new biologically inspired materials paradigm for environmental protection"
  • 2011 – Bridging the Gap, with collaboration with Dr P. Argondizza (Department of Music-Strathclyde U): "Orpheus underground"
  • 2011 – "Enhanced removal of nutrients, organic micropollutants, and toxicity from sewage lagoons" (collaboration with U. Manitoba and U. Winnipeg)
  • 2011 – EPSRC-Summer Vacation Bursary: "Tertiary treatment in constructed wetlands"
  • 2011 – 2014 PhD Scholarship: Unlocking sustainable sources of protein for animal feed
  • 2011 – 2014 PhD Scholarship (co-supervise): "Ecology of Legionella in compost"
  • 2010 – 2013 EPSRC-DTG (co-supervise): “Ecological recovery of previously contaminated soils"
  • 2009 – 2011 EPSRC (co-PI): “Bug-free prosthesis: reducing infection risk and improving reliability”, £201k.
  • 2009 – 2012 EPSRC-DTG: “Fate of antibiotic resistance genes upon exposure to heavy-metal pollution
  • 2009 Research Development Fund, "Fate of antibiotic resistance in sewage treatment", £6k

Knowledge Exchange & Industry Research

  • 2012 KTP - Scottish Canals (Co-investigator)
  • 2012 Scottish Funding Council, FAST (for accelerated sustainable transformations) microbes.
  • 2012 Scottish Funding Council, Landfuel: A feasibility study of reusing former landfills for energy using grass production (co-I)
  • 2012 Scottish Funding Council, YEAST: yield efficiency assessment and sustainability technologies.
  • 2011 Scottish Funding Council, algal biotechnology.
  • 2011 David Anderson Bequest funding - visiting researcher (Mark Hanson).
  • 2011 (Canada) visiting researcher to U. Manitoba and U. Winnipeg.
Publications

Cardinal P, JC Anderson, JC Carlson, JE Low, JK Challis, SA Beattie, CN Bartel, AD Elliot, SF Montero, S Lokesh, A Favreau, TA Kozlova, CW Knapp, ML Hanson, CS Wong 2014 Macrophytes may not contribute significantly to removal of nutrients, pharmaceuticals, and antibiotic resistance in model surface constructed wetlands. Science for the Total Environment 482/483: 294-304.

Ahmmad ZS, TR Sreekrishnan, CL Hands, CW Knapp, DW Graham 2014  Increased waterborne bla NDM-1 resistance gene abundances associated with seasonal human pilgrimages to the Upper Ganges River. Environmental Science & Technology 48(5): 3014-3020

Currie S, TK Beattie, CW Knapp, DSJ Lindsay 2013 Legionella spp. in UK composts – a potential public health issue. Clinical Microbiology and Infection 20(4): O224-9

Hughes M, S Debnath, CW Knapp, R Ulijn 2013 Antimicrobial properties of enzymatically triggered self-assembling aromatic peptide amphiphiles. Biomaterials Science. DOI:10.1039/C3BM60135H

Knapp CW 2013  (Book Review) "Horizontal Gene Transfer in Microorganisms, Edited by M. Pilar Francino." Ecotoxicology 22(9): 1443.

Knapp CW 2013  (Book Review) "Patricia L. Keen and Mark H. M. M. Montforts (eds.): Antimicrobial Resistance in the Environment." Ecotoxicology 22(4): 761-2

Anderson JC, JC Carlson, JE Low, JK Challis, CS Wong, CW Knapp, ML Hanson 2013 Performance of a constructed wetland in Grand Marais, Manitoba, Canada: removal of nutrients, pharmaceuticals, and antibiotic resistance genes from municipal wastewaterChemistry Central Journal 7:54.

CW Knapp, L Lima, S Olivares-Rieumont, E Bowen, D Werner, DW Graham 2012 Seasonal variations in antibiotic resistance gene transport in the Almendares River, Havana, Cuba. Frontiers in Microbiology 3: 396. doi: 10.3389/fmicb.2012.00396. 

Kates LN, C Gauchotte-Lindsay, N Nic Daéid, RM Kalin, CW Knapp, HE Keenan 2012 Prediction of the environmental fate of methylamphetamine waste. Environmental Forensics: Proceedings of the 2011 INEF Conference (book chapter): 262-74.

Ofiţeru ID, M Ferdeş, CW Knapp, DW Graham, V Lavric 2012 Conditional confined oscillatory dynamics of Escherichia coli strain K12-MG1655 in chemostat systems. Applied Microbiology and Biotechnology 94(1): 185-192.

Knapp CW, McCluskey SM, Singh B, Campbell CD, Hudson G, Graham DW 2011 Antibiotic resistance gene abundances correlate with metal and geochemical conditions in archived Scottish soils. PLoS One. 6(11).

McCluskey SM; CW Knapp 2011 Predicting antibiotic resistance... Frontiers in Microbiology 2:178.

El Ghazouani, A Basle, SJ Firbank, CW Knapp, J Gray, DW Graham, C Dennison 2011 Copper-binding properties and structures of methanobactins from Methylosinus trichosporium OB3b. Inorganic Chemistry 50(4): 1378-1391.

Graham DW; S Olivares-Rieumont; CW Knapp; L Lima; D Werner; E Bowen. 2011 Antibiotic resistance gene abundances associated with waste discharges to the Almendares river near Havana, Cuba. Environmental Science & Technology 45: 418-424.

Graham DW; C Trippett; WK Dodds; JM O'Brien; EBK Banner; IM Head; MS Smith; RK Yang; CW Knapp. 2010 Correlations between in situ denitrification activity and nir gene abundances in pristine and impacted prairie streams. Environmental Pollution 158: 3225-9.

Knapp, CW; J Dolfing; PAI Ehlert; DW Graham. 2010 Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940. Environmental Science & Technology, 44: 580-587.

Knapp, CW; W Zang; BSM Sturm; DW Graham. 2010 Differential fate of erythromycin and beta-lactam resistance genes from swine lagoon waste under different aquatic conditions. Environmental Pollution 158: 1506-1512.

Zhang, W.; BSM Sturm; CW Knapp; DW Graham. 2009 Accumulation of tetracycline resistance genes in aquatic biofilms due to periodic waste loadings from swine lagoons. Environmental Science & Technology, 43: 7643-7650.

Knapp, CW; WK Dodds; KC Wilson; JM O'Brien; DW Graham. 2009 Spatial heterogeneity of denitrification genes in a highly homogenous urban stream. Environmental Science & Technology, 43: 4273-4279.

Knapp, CW; DL Findlay; KA Kidd; DW Graham. 2008 A comparative assessment of molecular biological and direct microscopic techniques for assessing aquatic systems. Environmental Monitoring and Assessment, 145 (1-3): 465-473.

Knapp, CW; CA Engemann; ML Hanson; PL Keen; KJ Hall; DW Graham. 2008 Indirect evidence of transposon-mediated selection of antibiotic resistance genes in aquatic systems at low-level oxytetracycline Exposures. Environmental Science & Technology, 42 (14): 5348-5353.

Engemann, CA; PL Keen; CW Knapp; KJ Hall; DW Graham. 2008 Fate of tetracycline resistance genes in aquatic systems: Migration from the water column to peripheral biofilms. Environmental Science & Technology, 42 (14): 5131-5136.

Peak, N; CW Knapp; RK Yang; MM Hanfelt; MS Smith; DS Aga; DW Graham. 2007 Abundance of six tetracycline resistance genes in wastewater lagoons at cattle feedlots with different antibiotic use strategies. Environmental Microbiology, 9(1), 143-151.

Knapp, CW; DA Fowle; E Kulczycki; JA Roberts; DW Graham. 2007 Methane monooxygenase gene expression mediated by methanobactin in the presence of mineral copper sources. Proceedings of the National Academy of Sciences of the United states of America, 104(29), 12040-12045.

Kulczycki, E; DA Fowle; CW Knapp; DW Graham; JA Roberts. 2007 Methanobactin-promoted Dissolution of Cu-substituted Borosilicate Glass. Geobiology, 5, 251-263.

Knapp, CW; Graham, DW. 2007 Nitrite-oxidizing bacteria guild ecology associated with nitrification failure in a continuous-flow reactor. FEMS Microbiology Ecology, 62, 195-201.

Graham, DW; CW Knapp; ES Van Vleck; K Bloor;TB Lane; CE Graham. 2007 Experimental demonstration of chaotic instability in biological nitrification. The ISME Journal, 1(5), 385-393.

Hanson, ML; DW Graham; E Babin; D Azam; A-M Coutellec; CW Knapp; L Lagadic; T Caquet. 2007 Influence of isolation on the recovery of pond mesocosms from the application of an insectide: study design and planktonic community responses. Environmental Toxicology & Chemistry, 26, 1265-1279.

Hanson, ML; CW Knapp; DW Graham. 2006 Field assessment of oxytetracycline exposure to the freshwater macrophytes Egeria densa Planch. and Ceratophyllum demersum L. Environmental Pollution, 141, 434-442.

Engemann, CA; L Adams; CW Knapp; DW Graham. 2006 Disappearance of oxytetracycline resistance genes in aquatic systems. FEMS Microbiology Letters, 263, 176-182.

Cardoza, LA; CW Knapp, CK Larive, JB Belden, M. Lydy, and D.W. Graham. 2005 Factors affecting the fate of ciprofloxacin in aquatic field systems. Water Air and Soil Pollution, 161 (1-4), 383-398.

Knapp, CW, LA Cardoza; J Hawes; EMH Wellington; CK Larive; DW Graham. 2005 Fate and effects of enrofloxacin in aquatic systems under different light conditions. Environmental Science & Technology, 39, 9140-9146.

Chang, LW; GP Toth; DA Gordon; DW Graham; JR Meier; CW Knapp; J deNoyelles; S Campbell; DL Lattier. 2005 Responses of molecular indicators of exposure in mesocosms: common carp (Cyprinus carpio) exposed to the herbicides alachlor and atrazine. Environmental Toxicology & Chemistry, 24, 190-197.

Gordon, DA; GP Toth; DW Graham; JA Lazorchak; TV Redd; CW Knapp; F. deNoyelles; S Campbell; DL Lattier. 2005 Effects of eutrophication on vitellogenin gene expression in male fatehead minnows (Pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms. Environmental Pollution, 142 (3): 559-566.

Knapp, C.W, L. Lagadic, T. Caquet, M.L. Hanson, and D.W. Graham. 2005 Response of water column microbial communities to sudden exposure to deltamethrin in aquatic mesocosms. FEMS Microbiology Ecology, 54, 157-165.

Knapp, C.W. and D.W. Graham. 2004 Development of Alternate ssu-rRNA Probing Strategies for Characterizing Aquatic Microbial Communities. Journal of Microbiological Methods, 56, 323-330.

Smith, MS; RK Yang; CW Knapp; Y Niu; N Peak; M Hanfelt; JS Galland; DW Graham. 2004 Quantification of tetracycline resistance genes in feedlot lagoons by real-time PCR. Applied and Environmental Microbiology, 70, 7372-7377.

Ensz, AE, CW Knapp, DW Graham. 2003 Influence of Autochthonous Dissolved Organic Carbon and Nutrient Limitation on Alachlor Biotransformation in Aerobic Aquatic Systems. Environmental Science & Technology, 37 (18), 4157-4162.

Knapp, C.W.; Graham, D.W.; Steedman, R.J.; deNoyelles, F. 2003 Deep chlorophyll maxima in small boreal forest lakes after experimental catchment and shoreline logging. Boreal Environment Research, 8 (1), 9-18.

Knapp, C.W.; Graham, D.W.; Berardesco, G.; deNoyelles, F.; Cutak, B.J.; Larive, C.K. 2003 Nutrient level, microbial activity, and alachlor transformation in aerobic aquatic systems. Water Research, 37, 4761-4769.

Knapp, CW; F deNoyelles; DW Graham; S Bergin. 2003 Physical and chemical conditions surrounding the diurnal vertical migration of Cryptomonas spp. (Cryptophyceae) in a seasonally stratified midwestern reservoir (U.S.A.). Journal of Phycology, 39(5), 855-861.

Research students

Researchers

  • Jeremy Deeks (2011 - 2012*) Project Orpheus
  • Neil McCosh (2011 - 2012*) Underground nitrogen contamination

PhD

  • S Khan  The role of contaminated water in transferring antibiotic resistance
  • N Waddell (2nd supervisor) Increasing the capacity of earth filled flood embankments while minimising land use by examining commonly used assumptions. 
  • McCosh N  "Franken-soils"
  • Gkiouzepas S (2nd supervisor) Reduction of nitrates in groundwater
  • Currie S (2nd supervisor) Ecology of Legionella in compost.
  • Brennan A (2014*; 2nd supervisor) Ecotoxicology & fate of contaminants in biochar amended soils 
  • Catalina Silva-Plata (2nd supervisor) Socio-ecological resilience and water
  • Pape A (2014*) Geochemical changes, plant growth and ecosystem recovery in soils after high-temperature remediation.
  • McCluskey S  (2014*) Metal and antibiotic resistance in the environment.
  • Kates L (2013*; 2nd supervisor) Chemical profiling and environmental modelling of waste products from clandestine methylamphetamine laboratories.

MPhil

  • Curtis A Investigating phytoremediation following smoldering for contaminant removal 
  • Wade N Use of canal dredgings 

MRes

  • Miller R (2011*) Effect of ultrasound on plasmid mobility within different soil types
  • Sherrard T (2011*) Self-assembling bacteria population dynamics in industrial bioreactors
  • Skordou U (2012*; co-supervisor) Water flow & nutrient retention in smouldered soils

 

 * Graduated

Teaching activities:

Postgraduate duties:

  • MSc Env. Engineering - course director
  • International recruitment
  • Seminars: Advanced statistical analysis
  • Seminar: Dissertation workshop

Course registrar & lecturer:

  • EV908/CL431 - Pollution and Rehabilitation of Degraded Ecosystems (2012 - current)
  • CL948/CL430 - Principles of environmental microbiology (2011 - current)
  • CL978 - Wastewater & water treatment design

Contributing lectures:

  • CL420 - Water Engineering 2 (2013 - current)
  • BM304 - Microbiology - exploiting microbes (SIPBS)
Additional information/links:

Projects

  • A novel integrated ecological-geotechnical approach to management/adaptation of flood embankments

    Knapp, Charles (Co-investigator) Tarantino, Alessandro (Principal Investigator)

    Period 01-Oct-2013 - 30-Sep-2016
  • A novel integrated ecological-geotechnical approach to management/adaptation of flood embankments

    Knapp, Charles (Co-investigator) Tarantino, Alessandro (Principal Investigator)

    Period 01-Oct-2013 - 30-Sep-2016
  • KTP - Scottish Canals

    Knapp, Charles (Co-investigator) Switzer, Christine (Co-investigator) Lord, Richard (Principal Investigator)

    Period 22-Apr-2013 - 21-Apr-2015
  • Analysis of polychlorinated biphenyls (PCBs), dioxins and polycyclic aromatic hydrocarbons (PAHs) in tree cores using accelerated solvent extraction (ASE) and comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GCxGC-TOFMS)

    Knapp, Charles (Co-investigator) Kalin, Robert (Principal Investigator)

    Period 30-Jul-2012 - 30-Jan-2013
  • Orpheus Project

    Argondizza, Peter (Principal Investigator) Knapp, Charles (Principal Investigator)

    Period 01-Oct-2011 - 01-Jun-2012
  • Orpheus Project

    Argondizza, Peter (Principal Investigator) Knapp, Charles (Principal Investigator) Kalin, Robert (Academic)

    Period 01-Oct-2011 - 01-Apr-2012
  • BTG- Gene Transfer

    Knapp, Charles (Principal Investigator) Argondizza, Peter (Principal Investigator)

    Period 05-Sep-2011
  • Bug-free prostheses: Reducing infection risk and improving reliability

    Beattie, Tara (Co-investigator) Knapp, Charles (Co-investigator) Martin, Robert (Co-investigator) Meier, Margrit-Regula (Co-investigator) Sanchez, Marcelo (Co-investigator) Webb, Steven (Co-investigator) Lunn, Rebecca (Principal Investigator)

    Period 01-Nov-2009 - 30-Apr-2011

Course Coordinator

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