Dr Olaf Rolinski

Senior Lecturer



Resolving environmental microheterogeneity and dielectric relaxation in fluorescence kinetics of protein
Rolinski Olaf, Vyshemirsky Vladislav, McLaughlin Damien, Birch David
Methods and Applications in Fluorescence Vol 4, (2016)
Tyrosine photophysics during the early stages of β-amyloid aggregation leading to Alzheimer's
Rolinski Olaf J., Wellbrock Thorben, Birch David J. S., Vyshemirsky Vladislav
Journal of Physical Chemistry Letters Vol 6, pp. 3116-3120, (2015)
Birch David J. S., Chen Yu, Rolinski Olaf J.
Photonics: Scientific Foundations, Technology and ApplicationsPhotonics: Scientific Foundations, Technology and Applications, (2015)
Fluorescence kinetics of tryptophan in a heterogeneous environment
Rolinski Olaf J, Vyshemirsky Vladislav
Methods and Applications in Fluorescence Vol 2, (2014)
Inhibition of beta-amyloid aggregation by fluorescent dye labels
Amaro Mariana, Wellbrock Thorben, Birch David, Rolinski Olaf
Applied Physics Letters Vol 104, (2014)
CdSe/ZnS core/shell quantum dots as luminescence lifetime sensors for Cu2+
Sutter Jens, Birch David, Rolinski Olaf J.
Measurement Science and Technology Vol 23, (2012)

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Professional activities

Bit's 5th Annual Conference on Protein and Peptide
Invited speaker
12 International Conference on Methods and Applications of Fluorescence
Invited speaker
RSC, Chemistry World News Article
Invited seminar, Laboratory for Biomolecular Dynamics, Katolieke Universiteit Leuven, Belgium
Invited speaker
MAF 11
Invited speaker

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Doctoral Training Grant | Coulter, Jonathan
Birch, David (Principal Investigator) Rolinski, Olaf (Co-investigator) Coulter, Jonathan (Research Co-investigator)
Period 01-Oct-2009 - 19-Jun-2013
Doctoral Training Grant 2006 | McKendry, Jonathan
Dawson, Martin (Principal Investigator) Rolinski, Olaf (Co-investigator) McKendry, Jonathan (Research Co-investigator)
Period 01-Mar-2007 - 20-Apr-2011
EPSRC Doctoral Training Grant - DTA, University of Strathclyde | Mancini, Onorio Claudio
Mulheran, Paul (Principal Investigator) Rolinski, Olaf (Co-investigator) Mancini, Onorio Claudio (Research Co-investigator)
Period 01-Oct-2013 - 01-Apr-2017
Sol-gel pore nanomorphology using fluorescence resonance energy transfer
Birch, David (Principal Investigator) Rolinski, Olaf (Co-investigator)
We have measured for the first time the size of pores in the natural wet state during the formation of silica sol­gel ceramics using the quenching of fluorescent dyes which depends on the distance between the fluorescent dye and a quenching molecule. Our approach makes few assumptions, can be performed continuously in-situ and offers nanoscale resolution. The method is non-destructive and uses a pulsed source to stimulate fluorescence which then decays on the nanosecond timescale with a profile determined by the distribution of quenchers in the pore, thus reflecting the pore size. The pores are formed by the binding of silica nanoparticles and we have discovered a method of calibrating the particle size using standards and measuring it simply by means of the change in pH as the particles grow in size. The size of pores is found to typically have a radius in the region of a few nanometre (nm). The techniques we have developed are generic and can potentially be used on other porous solids as well as ceramics. Our findings are important stepping stones towards the goal of tracking and controlling the fundamental processes whereby a sol-gel ceramic is formed. This could well lead to the creation of new types materials with new physical and optical properties. The research contributed to the setting up of the joint Physics-Chemistry Centre for Molecular Nanometrology in Glasgow in 2005and subsequently helped attract the investment of a £5M Science and Innovation Award in nanometrology for research into molecular science, medicine and manufacture.

The project will provide a generic benefit by offering an improved approach to probing the in-situ morphology of wet porous solids in general, not just silica sol-gels, but also other ceramics such as clays, aluminosilicas, zeolites, shales, titania, sandstones and porous polymer resin supports etc. Specific benefits should accrue to the silica industry by better monitoring and control of the pore size in its products, enhancing product quality and reliability and to fluorescence lifetime instrument companies by the demonstration of a new application for such systems. Our research might also assist the production of new ceramics e.g. bio-compatible materials, photonic components, nano-powders etc where performance ultimately depends on measuring and controlling the nm morphology. Moreover, the determination of donor-acceptor distribution functions for fluorescence resonance energy transfer (FRET) in sol-gels should widen our understanding of this new approach to nano-structural determination and open up applications in other areas e.g. soft-solids such as liposomes, micelles and tissue and important bio-molecules such as proteins, indeed wherever FRET is used. Finally, there is a growing interest in understanding the photophysics of dyes in sol-gels in its own right for use in sensor systems.
Period 01-Feb-2004 - 30-Sep-2007
EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, David (Principal Investigator) Dawson, Martin (Co-investigator) Faulds, Karen (Co-investigator) Graham, Duncan (Co-investigator) Martin, Robert (Co-investigator) O'Donnell, Kevin (Co-investigator) Rolinski, Olaf (Co-investigator) Smith, William (Co-investigator) Wynne, Klaas (Co-investigator)
Capacity building in the area of nanometrology.
Period 01-Aug-2008 - 31-Jan-2012
Rolinski, Olaf (Principal Investigator) Birch, David (Co-investigator)
Metal ion sensing based on fluorescence lifetimes
Period 01-Oct-2006 - 31-Aug-2010

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