Dr Olaf Rolinski

Senior Lecturer

Physics

Publications

Cu2+ effects on beta‚Äźamyloid oligomerisation monitored by fluorescence of intrinsic tyrosine
Alghamdi Abeer, Wellbrock Thorben, Birch David JS, Vyshemirsky Vladislav, Rolinski Olaf J
ChemPhysChem (2019)
https://doi.org/10.1002/cphc.201900565
Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra
Chung Li Hung C, Birch David J S, Vyshemirsky Vladislav, Bella Angelo, Ryadnov Maxim G, Rolinski Olaf J
Methods and Applications in Fluorescence Vol 7 (2019)
https://doi.org/10.1088/2050-6120/ab1985
Tyrosine rotamer states in beta amyloid : signatures of aggregation and fibrillation
Mancini Onorio, Rolinski Olaf J, Kubiak-Ossowska Karina, Mulheran Paul A
ACS Omega Vol 3, pp. 16046-16056 (2018)
https://doi.org/10.1021/acsomega.8b02408
Probing beta amyloid aggregation using fluorescence anisotropy : experiments and simulation
Mancini Onorio, Wellbrock Thorben, Rolinski Olaf J, Kubiak-Ossowska Karina, Mulheran Paul A
Physical Chemistry Chemical Physics Vol 20, pp. 4216-4225 (2018)
https://doi.org/10.1039/C7CP08217G
Insulin aggregation tracked by its intrinsic TRES
Chung Li Hung C, Birch David J S, Vyshemirsky Vladislav, Ryadnov Maxim G, Rolinski Olaf J
Applied Physics Letters Vol 111 (2017)
https://doi.org/10.1063/1.5008477
Detecting beta-amyloid aggregation from the time-resolved emission spectra
Alghamdi Abeer, Vyshemirsky Vladislav, Birch David J S, Rolinski Olaf J
Methods and Applications in Fluorescence (2017)
https://doi.org/10.1088/2050-6120/aa9f95

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Professional activities

12th Fluorofest International Workshop
Participant
24/4/2017
Bit's 5th Annual Conference on Protein and Peptide
Invited speaker
23/3/2012
12 International Conference on Methods and Applications of Fluorescence
Invited speaker
11/9/2011
RSC, Chemistry World News Article
Contributor
2/2011
Invited seminar, Laboratory for Biomolecular Dynamics, Katolieke Universiteit Leuven, Belgium
Invited speaker
10/2009
MAF 11
Chair
9/2009

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Projects

EPSRC Doctoral Training Grant - DTA, University of Strathclyde | Mancini, Onorio Claudio
Mulheran, Paul (Principal Investigator) Rolinski, Olaf (Co-investigator) Mancini, Onorio Claudio (Research Co-investigator)
01-Jan-2013 - 09-Jan-2018
Doctoral Training Grant | Coulter, Jonathan
Birch, David (Principal Investigator) Rolinski, Olaf (Co-investigator) Coulter, Jonathan (Research Co-investigator)
01-Jan-2009 - 19-Jan-2013
EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, David (Principal Investigator) Dawson, Martin (Co-investigator) Faulds, Karen (Co-investigator) Girkin, John (Co-investigator) Graham, Duncan (Co-investigator) Martin, Robert (Co-investigator) O'Donnell, Kevin (Co-investigator) Rolinski, Olaf (Co-investigator) Smith, William (Co-investigator) Wynne, Klaas (Co-investigator)
The lack of capacity for advancing the emerging field of nanometrology can only be addressed through stra-tegic interdisciplinary collaborations that provide a stimulating and innovative research environment to catalyse and sustain a new dimension in UK research capability. In a major strategic initiative, Strathclyde University (SU) has founded a Centre for Molecular Nanometrology (2005 - to our knowledge, the first in the world) with facilities supported by the Wolfson Foundation and the Science Research Infrastructure Fund. This Centre has the ultimate goal of recording real-time images of dynamical interactions of single molecules in-situ. With the award of a Science and In-novation Award the Centre will facilitate the high quality, innovative, multidisciplinary research environment required to nurture and develop the extra capacity needed to make the UK a leader in nanometrology. A Science and Innovation Award will also bring together the Centre and medical collaborators at King's College London (KCL), bridging the molecular measurement gap to innovation in emerging areas of strategic impor-tance such as disease pathology, diagnostic tools in nanomedicine, the design of new drug treatments and new structural materials while facilitating knowledge transfer into the healthcare and chemical industries.
01-Jan-2008 - 31-Jan-2012
YOCTOMOLE FLUORESCENCE SENSING OF SINGLE METAL IONS
Rolinski, Olaf (Principal Investigator) Birch, David (Co-investigator)
Metal ions play both functional and toxic role in biological systems. Many enzymes require metal cations as co-factors, for example, Se, Zn and Ca, are essential components of glutathione peroxidase, insulin and calmodulin, respectively. Yet excessive Cu and Zn have deleterious effects in that they contribute to the oxidative stress and inflammation in the central nervous system. The presence of metal ions in our environment, e.g. surface waters, also has important influence on our health. Therefore, monitoring the levels of metal ions in living oranisms and the environment is a key analytical issue in life sciences with links to clinical medicine and in pollution control. At present, monitoring metal ions is mainly based on measurement of average concentration, which enables detection of 1 ion in the sample consisting of few billions of other molecules. We propose developing a new approach for single molecule detection, which would allow finding 1 ion in one mole of other molecules. This step improvement in sensitivity can offer a new insight into the research on ion interactions on molecular level, as it removes the complexity associated with ensemble-averaged macroscopic measurements. The goal can be achieved by combining single molecule detection techniques, enabling observation of the volume as small as ~1 micrometer^3, with fluorescence technique based on counting single photons. The aim is to develop the sensor to be able to recognise a specific single metal ion by using an ion-selective molecular mechanism, namely fluorescence resonance energy transfer (FRET).
01-Jan-2006 - 31-Jan-2010
Sol-gel pore nanomorphology using fluorescence resonance energy transfer
Birch, David (Principal Investigator) Rolinski, Olaf (Co-investigator)
01-Jan-2004 - 30-Jan-2007

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