Postgraduate research opportunities

Growth factor mediated trafficking of the tumour suppressor Sprouty-2

Cellular basis of disease, membrane trafficking

Number of places

1

Opens

21 February 2020

Deadline

31 March 2021

Duration

36 Months

Eligibility

Relevant qualification in biological course.

Project Details

Sprouty-2 is an important regulator of growth factor (FGF, EGF) signalling pathways and disruption of this protein is linked to cancer. Sprouty-2 has been shown to translocate from an intracellular pool to the plasma membrane upon growth factor stimulation. The minimal translocation domain of Sprouty-2 includes a highly palmitoylated cysteine-rich region, which regulates membrane interactions of Sprouty-2.

 

The movement of Sprouty-2 to the plasma membrane in response to growth factors is thought to be important for regulation of growth factor signalling, however there is very little known about the mechanisms controlling Sprouty-2 translocation or the role of palmitoylation in this process.

 

The aim of this project is therefore to define the trafficking pathway that mediates movement of Sprouty-2 to the plasma membrane in response to growth factors and to determine the importance of palmitoylation for this process.

 

 

Techniques used: The project will use a range of techniques including confocal microscopy, click chemistry and CRISPR.

Funding Details

Applicant will need to self-fund, find sponsorship for tuition and bench fees of £10,000 per annum for duration of studies

Supervisor

Primary Supervisor: Professor Luke Chamberlain

Email: luke.chamberlain@strath.ac.uk

 

Secondary Supervisor: Professor Gwyn Gould

Email: gwyn.gould@strath.ac.uk

Further information

Greaves J, Munro KR, Davidson SC, Riviere M, Wojno J, Smith TK, Tomkinson NC, Chamberlain LH. Proc Natl Acad Sci U S A. 2017 114(8):E1365-E1374.

Chamberlain LH, Shipston MJ. Physiol Rev. 2015 95(2):341-76

Contact us

Primary Supervisor: Professor Luke Chamberlain

Email: luke.chamberlain@strath.ac.uk