- Opens: Wednesday 20 May 2020
- Number of places: 1
- Duration: 36 Months
OverviewThe project will explore novel strategies to study cardiac cellular metabolism using isolated primary adult cardiac cells and the use of spheroids.
A pharmacological rat model of hypertension, cardiac hypertrophy and fibrotic remodelling will be used to explore changes in metabolism at the cellular level following cardiac remodelling. Primary adult cardiac myocytes and fibroblasts will be isolated from normal rats and rats subjected to chronic drug treatment. Drug-induced remodelling will also be performed on cells in culture in line with NC3Rs. Cells will either be maintained in culture or used on the day of isolation to investigate phenotypic and functional differences between cohorts. Key areas of focus will be on (i) intracellular calcium responses in both cell types (ii) mitochondrial function and motility and (iii) glucose transport. Intervention strategies will be explored that may either reduce or reverse metabolic impairment at a cellular level and this will also be investigated using the novel platform of cardiac multicellular spheroids.
Techniques to be used:
In vivo drug administration and echocardiography, immunohistochemistry, tissue and cell imaging, cell isolation, protein assays, quantitative immunoblotting, proliferation-based assays, calcium imaging and signalling-based assays, mitochondrial function and motility assays, glucose transport assays
Brand M.D. & Nicholls D.G. (2011) Biochem J. 435, 297-312
Szablewski L. (2017) Int.J.Cardiol. 230, 70-75
Polak-Iwaniuk A. et al (2019) Front.Physiol. 10:435
Strathclyde Institute of Pharmacy and Biomedical Sciences
Applicants can apply using the University PEGASUS Application System https://www.strath.ac.uk/science/strathclydeinstituteofpharmacybiomedicalsciences/studywithus-postgraduate/phd/