Dr Philipp Seib

Senior Lecturer

Strathclyde Institute of Pharmacy and Biomedical Sciences

Personal statement

RESEARCH OVERVIEW

Our research mission can be divided into bottom up, curiosity-driven fundamental research and top-down, challenge-based activities: the common thread to these activities are biomaterials, especially silk. Current research in the Seib lab spans the nano- to macro scale in the examination of silk for use in drug and cell delivery. 

Personal lab webpage is at:

www.SeibLab.com 

 

Silk-based drug delivery systems

We have a particular interest in exploring the use of silk for drug and cell delivery applications. Silk is an approved biopolymer for use in humans and has remarkable physical properties (e.g. it is tougher than any manmade fibre). Silk, a common suture material, has long been recognised for its biocompatibility and biodegradability. We are using it as a scaffold for tissue engineering and as a biopolymer for drug and cell delivery. Silk can be processed under mild aqueous conditions to generate several biomedically useful formats, including self-assembling silk hydrogels, nanoparticles, films and scaffolds.

 

Advanced drug delivery systems: the cellular response

We study the cellular response of drug delivery systems, including nanomedicines. Nanomedicines are specifically engineered, multiple-component, nanosized drugs and drug delivery systems whose use is emerging as a promising approach for treating many diseases, including cancer. The drug payloads of nanomedicines can differ widely, but the effectiveness of any nanomedicine relies on its ability to reach the tumour microenvironment. In addition, the nanomedicine often must deliver its drug payload to a specific internal cell compartment in order to yield the desired therapeutic effect. One of the bottlenecks for the translation of nanomedicines into clinical practice is the lack of suitable model systems for monitoring the cellular fate of nanomedicines, both in vitro and in vivo. Our laboratory focuses on the development of a repertoire of technologies for studying the cellular responses of nanomedicines. Our interests span the range from intracellular trafficking to application-oriented biocompatibility testing.

 

Engineering cellular microenvironments for stem and cancer cells

Our in vitro research focuses on overcoming the issues that arise when stem cells are studied in a highly artificial context that ignores their native microenvironment. The typical in vitro cell culture environment strips cells of most of the contextual signals and physical cues that arise from accessory cells and the extracellular matrix (ECM) found in intact tissues (e.g. tumours). The standard culture substrate for cells is treated polystyrene, which not only fails to mimic the complexity of the actual cell microenvironment, but also generates an artificial two-dimensional cell layer. We are developing a number of culture systems that better reflect the cellular microenvironments that occur in healthy and diseased tissues, including the three-dimensional space that stem cells normally occupy in a tissue.

We have recently extended the concept of an engineered stem cell microenvironment to three-dimensional scaffolds that are implanted in vivo and can serve as “traps” for circulating tumour cells. An emerging paradigm is that the stem cell niche can be hijacked by circulating tumour cells during the process of cancer metastasis. We are exploiting this feature to engineer artificial in vivo stem cell niches that selectively lure and trap cancer cells within the scaffold. The outcome of these studies will be unique new strategies for cancer therapy.

 

 

Publications

Silk hydrogel substrate stress relaxation primes mesenchymal stem cell behavior in 2D
Phuagkhaopong Suttinee, Mendes Luís, Müller Katrin, Wobus Manja, Bornhäuser Martin, Carswell Hilary V O, Duarte Iola F, Seib F Philipp
ACS Applied Materials and Interfaces (2021)
https://doi.org/10.1021/acsami.1c09071
Emerging silk material trends : repurposing, phase separation and solution-based designs
Seib F Philipp
Materials Vol 14 (2021)
https://doi.org/10.3390/ma14051160
Silk nanoparticle manufacture in semi-batch format
Matthew Saphia A L, Totten John D, Phuagkhaopong Suttinee, Egan Gemma, Witte Kimia, Perrie Yvonne, Seib F Philipp
ACS Biomaterials Science & Engineering (2020)
https://doi.org/10.1021/acsbiomaterials.0c01028
Focal drug administration via heparin-containing cryogel microcarriers reduces cancer growth and metastasis
Newland Ben, Varricchio Carmine, Körner Yvonne, Hoppe Franziska, Taplan Christian, Newland Heike, Eigel Dimitri, Tornillo Giusy, Pette Dagmar, Brancale Andrea, Welzel Petra B, Seib F Philipp, Werner Carsten
Carbohydrate Polymers Vol 245 (2020)
https://doi.org/10.1016/j.carbpol.2020.116504
Manual versus microfluidic-assisted nanoparticle manufacture : impact of silk fibroin stock on nanoparticle characteristics
Solomun Jana I, Totten John D, Wongpinyochit Thidarat, Florence Alastair J, Seib F Philipp
ACS Biomaterials Science & Engineering Vol 6, pp. 2796-2804 (2020)
https://doi.org/10.1021/acsbiomaterials.0c00202
Unraveling the impact of high-order silk structures on molecular drug binding and release behaviors
Wongpinyochit Thidarat, Vassileiou Antony D, Gupta Sukriti, Mushrif Samir H, Johnston Blair F, Seib F Philipp
Journal of Physical Chemistry Letters Vol 10, pp. 4278-4284 (2019)
https://doi.org/10.1021/acs.jpclett.9b01591

More publications

Professional activities

Frontiers in Bioengineering and Biotechnology (Journal)
Editorial board member
10/4/2021
ACS Nano (Journal)
Peer reviewer
10/4/2021
Advanced Functional Materials (Journal)
Peer reviewer
21/2/2021
Frontiers in Bioengineering and Biotechnology (Journal)
Peer reviewer
15/2/2021
India Alliance Welcome Trust (Grant reviewer)
Examiner
8/2/2021
Advanced Functional Materials (Journal)
Peer reviewer
15/1/2021

More professional activities

Projects

Biocompatible silk hydrogels as tunable stem cell constructs for the treatment of stroke. (skype)
Carswell, Hilary (Principal Investigator) Seib, Philipp (Co-investigator) Khadra, Ibrahim (Research Co-investigator)
01-Jan-2020 - 30-Jan-2023
Microfluidic-assisted manufacture of self-assembling silk nanoparticles
Seib, Philipp (Principal Investigator) Perrie, Yvonne (Co-investigator)
01-Jan-2019 - 30-Jan-2023
Engineered substrates for the isolation and expansion of mesenchymal stem cells
Seib, Philipp (Principal Investigator)
01-Jan-2019 - 30-Jan-2022
EPSRC Centre for Doctoral Training in Future Power Networks and Smart Grids | Egan, Gemma
Connolly, Patricia (Principal Investigator) Seib, Philipp (Co-investigator) Egan, Gemma (Research Co-investigator)
01-Jan-2017 - 01-Jan-2022
Self-assembling silk hydrogels
Seib, Philipp (Principal Investigator)
01-Jan-2017 - 31-Jan-2020
Engineered culture surfaces for the isolation and expansion of bone fide mesenchymal stem cells (MSCs)
Seib, Philipp (Principal Investigator)
Engineered culture surfaces for the isolation and expansion of bone fide mesenchymal stem cells(MSCs)
01-Jan-2017 - 28-Jan-2018

More projects

Address

Strathclyde Institute of Pharmacy and Biomedical Sciences
Robertson Wing

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