PAC Mass SpectrometryStandard Mass Spectrometry Analysis

The PAC Mass Spectrometry Facility offers walk up services for routine chemical analysis of molecules such as novel synthesised compounds, proteins and peptides at various stages of research: Reaction Mixtures, Digests from Biological materials, Purified isolates to name a few.

Training can be provided upon request for the following techniques:

  • Liquid Chromatography-Mass Spectrometry (Agilent LCMS and Advion LCMS)
  • Gas Chromatography-Mass Spectrometry(Agilent GCMS)
  • Gas Chromatography-Flame Detection Ionisation (Agilent GC-FID)
  • TLC Plate Reader/Direct Injection/Mass Spectrometry (Advion TLC Plate Reader)

Agilent LCMS

Agilent LCMS Mass Spectrometry

The Agilent LCMS is the primary workhorse of the facility for reaction monitoring and verification of synthesis success. The system has a quad-pump so supports easy switching between two solvent gradients using Acetonitrile/Water with 0.1% Formic Acid or 5mM Ammonium Acetate buffer.

Specifications:

Agilent Technologies 1200 Series HPLC
Agilent Technologies 6130 Quadrupole

Ionisation: Combined ESI/APCI
Detectors: Single wavelength UV and Quadrupole Mass Spectrometer 
Column: Agilent Infinity Lab Poroshell 120, 4.6x100mm, 2.7 micron C18

Advion LCMS

Advion LCMS Mass Spectrometry

The Advion LCMS is a UPLC-MS setup allowing for faster run times than conventional HPLC seperations as well as more defined chromatographic seperation of mixtures. While this instrument does have standard methods for routine analysis, this instrument can also be optimised for a researcher's specific analysis needs upon request such as changing columns or use of new solvent systems.

Specifications:

Advion Expression L Compact Mass Spectrometer (CMS)
Advion Avant Series UPLC (A-2155,A-2112,A-2046)

Ionisation: ESI
Detectors: Multi-Wavelength UV and Mass Spectrometer
Standard Solvent System for Walk-Up Use: Water/Acetonitrile using either 5mM Ammonium Acetate or 0.1% Formic Acid as Buffer
Column Installed for Walk-Up Use:Phenomenex Kinetex 30x2.1mm 1.7um C18

Advion TLC/Direct Injection MS

Advion TLC Plate Reader Mass Spectrometer

The Advion TLC/ Direct Injection MS is for samples from in progress work, getting a quick analysis of one's sample under 5 minutes. TLC plates can be analysed spot by spot by the Mass Spectrometer or samples can be directly injected into the ion-source.

 

 

 

Specifications:

Advion Plate Express
Advion Expression L Compact Mass Spectrometer

Ionisation: ESI (APCI on request)
Detector: Single Quadrupole Mass Spectrometer

Agilent GCMS

Agilent GCMS

This Technique is idea for separating and identifying components in volatile and semivolatile organic complex mixtures in the gas phase. This set up is attached to a NIST Library allowing for the identification of compounds against verified GCMS results.

 

 

 

Specifications:

Agilent Technologies 7890A Gas Chromatography
Agilent Technologies 5975C inert XL EI/CI MSD with Triple-Axis Detector

Ionisation: Electron Impact
Column:Restek 30mx0.25mmidx0.25um
Mobile Phase: Helium
Detector: Monolithic hyperbolic Quadropole Mass Spectrometer

Agilent GC-FID

GC FID

The Agilent GC-FID is for the analysis of small volatile compounds. Using a Flame Ionisation Detector instead of a Mass Spectrometer for detection, this system is choice for determination of sample purity from synthesis of small organic compounds. The system is set up to mirror the gradients found on the GCMS across from it in the Facility.

Specifications:

Agilent Technologies 7890A Gas Chromatograph

Column: Restek 30mx0.25mmidx0.25um
Carrier Gas: Helium
Detector: Flame Ionisation Detector